Analysis of efficacy of different treatment modalities and prognostic factors in patients with locally advanced Masaoka-Koga stage Ⅲ thymoma / 中华放射肿瘤学杂志

Objective:To analyze the clinical efficacy of different treatment modalities and prognostic factors of patients with Masaoka-Koga stage Ⅲ thymoma.Methods:Clinical data of patients diagnosed with Masaoka-Koga stage Ⅲ thymoma admitted to Affiliated Cancer Hospital of Zhengzhou University from January 2000 to December 2018 were analyzed retrospectively. A total of 133 patients had complete treatment and follow-up data. Kaplan-Meier method was used to calculate the cumulative survival rate, log-rank method was used to compare the survival between two groups, and Cox regression model was used for multivariate analysis.Results:The median follow-up time was 50 months (3-221 months). The median overall survival (OS) was 51 (3-221) months, and the median disease-free survival (DFS) was 45 (2-221) months. The survival rate in the radical surgery group was better than that in the palliative surgery group. The 5- and 10-year OS rates in radical surgery group were 88.2% and 74.4% respectively, while in palliative surgery group were 51.8% and 32.4% respectively ( P<0.001). The 5- and 10-year DFS rates in radical surgery group were 72.2% and 45.5%, respectively, while in palliative surgery group were 32.3% and 16.1% respectively ( P=0.001). The OS in the surgery combined with radiotherapy group was better than that in the surgery alone group. The 5- and 10-year OS rates in the radical surgery group were 82.8% and 64.2% respectively, while in the palliative surgery group were 55.8% and 50.2% ( P=0.033). There was no significant difference in DFS between two groups ( P=0.176). Multivariate analysis showed that age < 50 years old ( HR=0.264, P=0.001), radical resection ( HR=0.134, P<0.001), surgery combined with radiotherapy ( HR=2.778, P=0.009) were independently associated with better OS. Age < 50 years old ( HR=0.550, P=0.046), radical resection ( HR=0.555, P=0.042), and invasion of single organ ( HR=0.111, P=0.003) were independently associated with better DFS. Conclusions:OS and DFS in patients undergoing radical surgery are significantly better than those in their counterparts treated with palliative surgery, which is the most important factor affecting prognosis. Surgery combined with radiotherapy yields better OS. It is necessary to design a rigorous and reasonable multicenter prospective study to evaluate the efficacy of various treatment modalities and prognostic factors.

Analysis and prospect of potential value of fecal microbiota transplantation in the treatment of short bowel syndrome / 中国综合临床

Short bowel syndrome (SBS) is a series of clinical syndromes caused by the reduction of effective functional area of small intestine for different reasons.Due to the changes of intestinal function and internal environment, SBS can further lead to intestinal mucosal barrier damage, enterogenous liver damage, abnormal bile acid metabolism and so on, which seriously affects the clinical outcome and prognosis of patients. Fecal microbiota transplantation is the transplantation of normal functional bacteria in feces into patients' intestines to help restore and rebuild the balance of intestinal flora. It has been successfully used in the treatment of intestinal and extraintestinal diseases. This paper reviews and prospects the potential value of fecal bacteria transplantation in the treatment of SBS for D-lactic acidosis, intestinal rehabilitation and liver damage associated with intestinal failure, in order to provide new clinical ideas for the treatment of SBS patients.

Applying the adjustment set to estimate causal effect of data based on the causal graph model / 四川精神卫生

The purpose of this paper was to introduce the five limitations of the PROC CAUSALGRAPH procedure and estimate the causal effect of the data by using the adjustment set based on the causal graph model. The five limitations were as follows： ①the PROC CAUSALGRAPH procedure could not deal with the causal graph model of directed circles； ② the PROC CAUSALGRAPH procedure could not evaluate dynamic processing scheme； ③ causal effect identification was a population concept； ④ causal effect identification was a nonparametric concept； ⑤ the PROC CAUSALGRAPH procedure could not identify the causal effect in some causal graph models. The example was for a simulated data set， using the conventional multiple Logistic regression model analysis and the causal graph model analysis， respectively. By comparing the analysis results of the two， the following conclusions were drawn： ① causal graph theory was useful in identifying causal effects in confounding situations； ② by implementing hierarchical estimation of causal effects， a good statistical estimation of causal effects could be achieved based on the identification results of the PROC CAUSALGRAPH procedure.

Reasonably conduct the multiple Logistic regression analysis combined with the average treatment effect analysis / 四川精神卫生

The purpose of the paper was to introduce how to reasonably carry out multiple Logistic regression analysis combined with the average treatment effect analysis. Firstly， it introduced 4 basic concepts related to the average treatment effect analysis. Secondly， it presented the core contents in the average treatment effect analysis， that was， six estimation methods. Thirdly， through a hypothetical drug clinical trial example， it gave the whole process of how to use SAS software for the analysis. The contests were as follows： ① the traditional multiple Logistic regression model was used for the analysis； ② the propensity score model was used to calculate the inverse probability weights； ③ six estimation methods were used to estimate the potential outcome mean and the average treatment effect.

Comparison of clinical features of Takayasu′s arteritis in children at different ages / 中华实用儿科临床杂志

Objective:To improve the understanding of Takayasu′s arteritis (TA) and its diagnosis and treatment by analyzing and summarizing the clinical characteristics of TA in children at different ages.Methods:Clinical and follow-up data of 41 children with TA admitted in Children′s Hospital, Capital Institute of Pediatrics between January 2010 and May 2020 were retrospectively analyzed.Based on the cut-off age of 3 years, children with TA were divided into older group and younger group.Clinical characteristics, involvement of the coronary artery, blood pressure control and growth restriction between 2 groups were analyzed.Counting data were expressed as percentage and case, and compared by the Chi- square test. Results:Among the 17 children with TA in younger group, there were 8 males and 9 females.There were 6 males and 18 females in older group.The general type was most common in younger group, with 10 cases (58.8%). In older group, thoracic and abdominal aortic type was the most common, with 13 cases (54.2%). The most common clinical manifestation in younger group was fever, with 13 cases (76.5%). In older group, 19 patients (79.2%) had hypertension.Lower hemoglobin (Hb) was detected in younger group.Leukocyte count, C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR) increased in all patients (100.0%). In older group, leukocyte count increased in 6 cases (25.0%), ESR increased in 13 cases (54.2%), and CRP increased in 11 cases (45.8%). The coronary artery and its branches (anterior descending branch and circumvolute branch) were the mostly affected in younger group, with 16 cases (94.1%). The subclavian artery was the most commonly involved in older group (15 cases, 62.5%). All TA children in younger group were in the active stage.Among them, 8 cases were treated with biological agents alone, 3 cases were treated with glucocorticoid alone, 5 cases were treated with the combination of glucocorticoid and biological agents, and 1 case was treated with glucocorticoid first, and then transferred to biological agents due to the poor effect.In older group, there were 18 active-stage patients (75.0%), and 2 refused treatment.Sixteen active patients and 6 inactive patients were treated with glucocorticoid, involving 19 cases treated with glucocorticoid combined with Cyclophosphamide, and 3 cases treated with glucocorticoid combined with biologics.There were 16 cases of coronary artery involvement in younger group and only 1 case in older group ( P<0.01). In younger group, 9 patients had growth restriction, while none was detected in older group ( P<0.01). The blood pressure of younger group was all controlled, which was not satisfactorily controlled in 16 cases of older age ( P<0.01). The incidence of general type and active stage in younger group was higher than that of older group without significant difference ( P>0.05). Conclusions:The clinical characteristics of TA vary at different ages.TA progresses more rapidly in younger children, which are more prone to the involvement of extensive vessels, the coronary arteries and other vessels, and the effects of drugs on growth and development should be well concerned.Older TA patients can be alleviated into the inactive phase by themselves, which is mainly characterized as the involvement of large vessels and hypertension sequelae.

Correlations between genetic polymorphism of IFN-λ family gene and HBV infection, virus replication and clearance / 生物工程学报

Hepatitis B virus (HBV) infection is one of the most serious public health problems. HBV infection could lead to hepatitis B, and even further develop into hepatic cirrhosis and hepatocellular carcinoma. Interferon lambda (IFN-λ) is a member of the interferon (IFN) family and an important cytokine for antiviral defense. There are four members in IFN-λ family, including IFN-λ1, IFN-λ2, IFN-λ3, and IFN-λ4. The genetic polymorphisms in the IFN-λ genes are associated with HBV replication and treatment response of HBV patients. In this review, we summarized the roles of genetic polymorphisms of the IFN-λ genes played in HBV infection, disease progression and treatment, with the aim to better understand their function. This review could serve as a reference for the HBV prevention and treatment of HBV patients, as well as for future clinical usage.

Therapeutic effect of artificial liver multi-mode sequential combination in patients with hepatitis B virus-related acute-on-chronic liver failure / 中华传染病杂志

Objective:To explore the therapeutic effect of multi-mode sequential combination of artificial liver in the treatment of hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF).Methods:The clinical data of HBV-ACLF patients treated with artificial liver in Wuxi Fifth People′s Hospital from January 2018 to June 2021 were retrospectively analyzed. Eighty-six patients were divided into artificial liver multi-mode sequential combination therapy group (sequential combination group) and conventional treatment group. The cytokine level changes and model for end-stage liver disease (MELD) score were analyzed at 14 days of disease duration. The survival outcome and complications of artificial liver were analyzed after 30 days of follow-up. Two independent samples t test and chi-square test were used for statistical analysis. Cox regression analysis was used to analyze the risk factors of death, and Kaplan-Meier method was used to analyze the survival rate of patients. Results:A total of 86 patients were enrolled, including 48 patients in sequential combination group with the average number of artificial liver of 4.68 times/person, and 38 patients in conventional treatment group with the average number of artificial liver of 3.17 times/person. At 14 days of disease duration, interleukin (IL)-6, IL-8, interferon γ-inducible protein (IP)-10 level and MELD score in sequential combination group decreased significantly than those in the conventional treatment group ( t=3.80, 3.62, 4.95 and 1.11, respectively, all P<0.050). After 30 days of follow-up, 63 patients survived and 23 patients died. Cox regression analysis showed that baseline international normalized ratio (hazard ratio ( HR)=0.558, 95% confidence interval ( CI) 0.193 to 0.856, P=0.027), baseline antithrombin Ⅲ activity ( HR=0.876, 95% CI 0.824 to 0.932, P<0.001), artificial liver mode ( HR=0.819, 95% CI 0.236 to 0.992, P=0.005), spontaneous peritonitis ( HR=0.170, 95% CI 0.045 to 0.647, P=0.009) and hepatic encephalopathy ( HR=0.004, 95% CI 0.001 to 0.030, P<0.001) were independent influencing factors for 30-day survival outcome. The cumulative survival rate of sequential combination group was higher than that of conventional treatment group, and the difference was statistically significant ( χ2=5.45, P=0.020). There were no significant differences in the proportions of bleeding, deep vein thrombosis, heart rate and blood pressure instability between the two groups ( χ2=0.63, 1.20 and 0.54, respectively, all P>0.050). The platelet decline of patients in sequential combination group was slighter than that in conventional treatment group, and the difference was statistically significant ( t=-4.17, P=0.002). Conclusions:Multi-mode sequential combination therapy of artificial liver could eliminate cytokines and reduce MELD score more effectively in patients with HBV-ACLF, and prolong the survival time of patients and have little effect on platelet count.

Effect of botulinum toxin type a in congenital muscular torticollis / 中华医学美学美容杂志

Objective:To discuss the clinical results of treatment for congenital muscular torticollis with different injection points of botulinum toxin type A and traditional conservative method, and to expound the safety and effectiveness of this way.Methods:From January 2018 to December 2018, 60 cases aged from one month to six months with congenital muscular torticollis who visited the outpatient clinic of plastic surgery in Children＇s Hospital of Quanzhou, a teaching hospital of FuJian Medical University, and there were 38 males and 22 females. The treatment groups were divided into two groups according to random number method that were treated with botulinum toxin type A: the single point group was given one point injection, and the three-point group was injected with three points, while the control group was treated with traditional conservative treatment (mainly manual stretching exercises). The number of cases in each group was 20.Results:The differences of muscle thickness and muscle length were repeatedly measured at different time points in the same group and variance analysis conducted. The results showed that there were statistical significances among the two indicators at different time points in each group ( P<0.05). Least significant difference (LSD) was further adopted for pairwise comparison between indicators at different time points in each group and the differences were statistically significant ( P<0.05). In the comparison of treatment effect of different groups after twelve months follow-up, the cure rate was 85% (17 cases) in the single point group, 95 % (19 cases) in the three-point group, and 80 % (16 cases) in the control group, there was no significant difference among three groups ( P>0.05). Conclusions:Local injection of botulinum toxin type A is a safe and effective treatment option for congenital muscular torticollis, which can achieve the same clinical effect as traditional conservative treatment.

Two transfusion strategies for severe burn patients in non-shock stage: A comparative study / 中国输血杂志

【Objective】 To investigate the effect of two different blood transfusion strategies in non-shock stage of sever burn patients, so as to provide reference for clinical rational use of blood. 【Methods】 74 cases of severe burn patients in our hospital from July 2019 to December 2020 were randomly divided into restrictive transfusion group and liberal transfusion group. The differences of blood transfusion volume, red blood cell (RBC) count before and after blood transfusion, Hb value, incidence of transfusion adverse reactions, postoperative infection rate, length of hospital stay, and 30 day mortality between the two groups were retrospectively analyzed. 【Results】 1) The blood transfusion volume of the restricted blood transfusion group [(9.58±7.43)U] was statistically less than that of the liberal blood transfusion group [(22.24±20.08)U] (P 0.05). 【Conclusion】 Limited blood transfusion treatment for severe burn patients in non-shock stage can reduce the frequency and risk of blood transfusion and save blood resources, which is of great significance for rational and safe blood use in clinic.

Clinical effect of Kangfu Xiaoyan suppository combined with moxifloxacin in the treatment of chronic pelvic inflammatory disease / 中国基层医药

Objective@#To explore the clinical effect of Kangfu Xiaoyan suppository combined with moxifloxacin in the treatment of chronic pelvic inflammatory disease.@*Methods@#From November 2016 to November 2018, a total of 216 patients with chronic pelvic inflammatory disease admitted to the Maternal and Child Health Care Hospital of Lishui were enrolled.The patients were divided into control group and observation group according to the digital table, with 108 cases in each group.The patients in the control group were treated with quinolone antibacterial agent moxifloxacin hydrochloride tablets.The patients in the observation group were treated with Kangfu Xiaoyan suppository and moxifloxacin hydrochloride tablets.The disappearance time of clinical signs, clinical treatment effects and adverse reactions in the two groups after treatment were observed and compared.@*Results@#The disappearance time of fever[(5.15±2.46)h], lower abdominal pain[(7.62±2.77)h], leucorrhea abnormality[(9.16±3.13)h], and pain[(6.38±2.03)h] in the observation group were significantly shorter than those in the control group[(8.09±2.28)h, (10.45±2.68)h, (12.20±2.96)h, (9.49±2.27)h](t=8.231, 7.631, 7.334, 10.613, all P=0.000). The total effective rate of the observation group (93.51%) was significantly higher than that of the control group (86.11%), the difference was statistically significant (χ2=6.983, P=0.008). The overall incidence of adverse reactions of the observation group (3.70%) was significantly lower than that of the control group (11.11%), and the difference was statistically significant (χ2=4.32, P=0.038).@*Conclusion@#Compared with the simple use of moxifloxacin, Kangfu Xiaoyan suppository combined with moxifloxacin in the treatment of gynecological chronic pelvic inflammatory disease has a better clinical treatment effect and a lower incidence of adverse reactions.It is recommended to apply in clinical treatment in the future.

Analysis of the effect of endoscopic surgery combined with conventional surgery in Neuroblastoma / 临床耳鼻咽喉头颈外科杂志

The aim of this study is to evaluate the efficacy of endoscopic surgery and conventional surgery combined with radiotherapy in the treatment of Neuroblastoma. Forty-three patients with olfactory neuroblastoma undergoing surgery combined with radiotherapy were retrospectively analyzed. The patients were divided into endoscopic surgery and conventional surgery. All patients received postoperative radiotherapy at a dose of 60-70 Gy, the 5-year survival rate and local recurrence time of the two groups were compared, and the therapeutic effects of endoscopic surgery and traditional surgery were compared. Through survival analysis, the 5-year overall survival rates of the traditional surgery group and the endoscopic surgery group were 50% and 58% （=0.560）, the local recurrence rates were 44% and 48% （=0.288）, and the mean recurrence time was 5.6 months and 12.5 months （=0.032）. There was no difference between endoscopic surgery and conventional surgery combined with radiotherapy in the treatment of Neuroblastoma, and the time of local recurrence was significantly prolonged. In early Neuroblastoma, endoscopic sinus surgery may be superior to open surgery in terms of efficacy and patient survival.

Analysis of the effect of endoscopic surgery combined with conventional surgery in Neuroblastoma / 临床耳鼻咽喉头颈外科杂志

Objective@#The aim of this study is to evaluate the efficacy of endoscopic surgery and conventional surgery combined with radiotherapy in the treatment of Neuroblastoma. @*Method@#Forty-three patients with olfactory neuroblastoma undergoing surgery combined with radiotherapy were retrospectively analyzed. The patients were divided into endoscopic surgery and conventional surgery. All patients received postoperative radiotherapy at a dose of 60-70 Gy, the 5-year survival rate and local recurrence time of the two groups were compared, and the therapeutic effects of endoscopic surgery and traditional surgery were compared.@*Result@#Through survival analysis, the 5-year overall survival rates of the traditional surgery group and the endoscopic surgery group were 50% and 58% （P=0.560）, the local recurrence rates were 44% and 48% （P=0.288）, and the mean recurrence time was 5.6 months and 12.5 months （P=0.032）.@*Conclusion@#There was no difference between endoscopic surgery and conventional surgery combined with radiotherapy in the treatment of Neuroblastoma, and the time of local recurrence was significantly prolonged. In early Neuroblastoma, endoscopic sinus surgery may be superior to open surgery in terms of efficacy and patient survival.

Clinical effect of Kangfu Xiaoyan suppository combined with moxifloxacin in the treatment of chronic pelvic inflammatory disease / 中国基层医药

Objective To explore theclinical effect of Kangfu Xiaoyansuppositorycombined with moxifloxacin in the treatment of chronic pelvic inflammatory disease.Methods From November 2016 to November 2018,a total of 216 patients with chronic pelvic inflammatory disease admitted to the Maternal and Child Health Care Hospital of Lishui were enrolled .The patients were divided into control group and observation group according to the digital table , with 108 cases in each group .The patients in the control group were treated with quinolone antibacterial agent moxifloxacin hydrochloride tablets .The patients in the observation group were treated with Kangfu Xiaoyan suppository and moxifloxacin hydrochloride tablets .The disappearance time of clinical signs ,clinical treatment effects and adverse reactions in the two groups after treatment were observed and compared .Results The disappearance time of fever [(5.15 ±2.46)h],lower abdominal pain[(7.62 ±2.77)h],leucorrhea abnormality[(9.16 ±3.13)h],and pain [(6.38 ±2.03) h] in the observation group were significantly shorter than those in the control group [(8.09 ± 2.28)h,(10.45 ±2.68)h,(12.20 ±2.96)h,(9.49 ±2.27)h](t=8.231,7.631,7.334,10.613,all P=0.000). The total effective rate of the observation group (93.51％) was significantly higher than that of the control group (86.11％),the difference was statistically significant (χ2 =6.983,P=0.008).The overall incidence of adverse reactions of the observation group (3.70％) was significantly lower than that of the control group (11.11％),and the difference was statistically significant (χ2 =4.32,P =0.038).Conclusion Compared with the simple use of moxifloxacin ,Kangfu Xiaoyan suppository combined with moxifloxacin in the treatment of gynecological chronic pelvic inflammatory disease has a better clinical treatment effect and a lower incidence of adverse reactions .It is recommended to apply in clinical treatment in the future .

Estimation on the individual treatment effect among heterogeneous population, using the Causal Forests method / 中华流行病学杂志

Objective@#This project aimed to explore the effectiveness of estimating individual treatment effect on real data, among the heterogeneous population, with Causal Forests (CF) method, to find out the characteristics of heterogeneous population.@*Methods@#We designed and conducted four computer simulation schemes to verify the effect of estimating on individual treatment, using the CF under four different environments of the treatment effects. Real data was then analyzed for the catheterization on right heart.@*Results@#Results from the simulation process showed that the values on individual treatment effect that were estimated by causal forests were consistent with the population effect as well as in line with the expected distribution under the setting of four different effect values. Results of real data analysis showed that values of individual treatment effect among most patients appeared positive, so the use of RHC could cause an increase of the '180-day mortality rate’ in the sampled population. Patients with lower predicted probability of 2-mo survival and albumin were more likely to have a lower risk of death after using the RHC.@*Conclusion@#CF method could be effectively used to estimate the individual treatment effect and helping the individuals to make decision on the receipt of treatment.

Effect of massage combined withwarm acupuncture on early primary frozen shoulder / 中国基层医药

Objective@#To observe the clinical effect of massage combined with warm acupuncture and moxibustion on primary frozen shoulder.@*Methods@#From April 2018 to April 2019, 30 patients with primary frozen shoulder admitted and treated in Wenzhou Hospital of Traditional Chinese Medicine were selected in theresearch.According to different admission time, they were divided into observation group and control group, with 15 patients in each group.The control group was treated with warm acupuncture and moxibustion, while the observation group was combined with massage.The total effective rate and VAS pain score after treatment were compared and observed.@*Results@#The total effective rate in the observation group was 93.33%, which was higher than 73.33% in the control group (χ2=3.294, P<0.05). The VAS score of the observation group was (1.05±0.22)points after treatment, which was lower than (3.13±0.43)points of the control group, the difference between the two groups was statistically significant(t=2.493, P=0.000).@*Conclusion@#Massage combined with warm acupuncture and moxibustion in the treatment of primary frozen shoulder is more effective than the single treatment method, which can relieve the pain of patients and is a safe and effective treatment.

Aesthetic effect of minimally invasive therapy on stained dental fluorosis / 中华地方病学杂志

Objective To observe the aesthetic effect of low concentration of at-home bleaching combined with microabrasion and resin infiltration on stained dental fluorosis.Methods Twenty-three patients with stained dental fluorosis were selected from June 2017 to June 2018 in Stomatological Hospital of Tianjin Medical University.All patients were treated with minimally invasive whitening technology of microabrasion,10％ low concentration of at-home bleaching and resin infiltration.The aesthetic treatment effect was observed before and after treatment by VITA aesthetic shade guide matching.Stained spots were chosen and colorimetric assay was performed using CMYK shade guide before treatment.According to the color of the splash,the light and shade were divided into light grade,medium grade and heavy grade.The CMYK data of the selected spot and complete decolorization ratio (stain decolorization rate) were recorded 1 week and half a year after treatment.Meanwhile,tooth sensitivity was recorded using visual analogue scale (VAS).Results The shades of 92 teeth of 23 dental fluorosis patients were increased more than 5 degrees after a series of whitening treatment,1 tooth was 2-4 degrees,and the total effective rate was 100.00％ (92/92).Decolorization rates of light grade,medium grade and heavy grade were 100.00％ (100/100),100.00％ (120/120) and 97.78％ (88/90),respectively,1 week after treatment.Half a year after treatment,bleaching decolorization rate had no significant difference compared with that of 1 week after treatment (P ＞ 0.05).Tooth sensitivity was 91.30％ (21/23).Almost all patients had mild and moderate teeth sensitivity during treatment.The symptoms of tooth sensitivity were disappeared after the treatment.Conclusions The whitening technology of microabrasion,10％ low concentration of at-home bleaching and resin infiltration has a significant decolorization whitening effect,and has a certain degree of stability and no sensitive teeth after treatment.It is worthy of clinical application.

Preventive effect and mechanism of puerarin on rat models of disuse osteoporosis / 中国中药杂志

To investigate the preventive effect and possible mechanism of puerarin(Pur) in rat model of disuse osteoporosis(DOP),thirty healthy Wistar female rats of 2 months old were randomly divided into control group(Control), hindlimb suspension group(HLS), and puerarin group(HLS+Pur) in hindlimb suspension, with 10 rats in each group. A disuse osteoporosis model was established by tail suspension method, and 15.4 mg·kg~(-1) puerarin suspension was administered to HLS+Pur group every day, and the same volume of distilled water was administered to Control group and HLS group respectively. After 28 days, the rats were sacrificed by abdominal aorta blood collection, the main organs of the rats were removed, and the bone tissues of the rats were dissected. The organ index of the rats was calculated and the histopathology of the organs was observed under microscope. Bone mineral density test and bone biomechanical experiment were performed. Bone histomorphometry results were observed after bone tissue sectioning, and serum biochemical markers of bone metabolism were determined. There was no significant difference in organ index between the groups. There was no obvious abnormality in the pathological examination of the organs. The results of bone mineral density showed that puerarin could significantly increase the bone density of the tibia and vertebrae caused by hindlimb suspension. The mechanical parameters experiments showed that puerarin could effectively increase the maximum load and elastic modulus of the tibia and vertebrae. Fluorescence labeling showed that the fluorosis interval increased and the bone formation increased during puerarin treatment. The VG staining results showed that compared with the HLS group, in the puerarin group, the number of trabecular bone increased, the thickness of the trabecular bone became thicker, and the bone separation became smaller, which greatly improved the bone microstructure after hindlinb suspension. In addition, serum biochemical indicators showed that puerarin could promote bone formation index bone calcium. The content of osteocalcin(OC) increased and inhibited the formation of tartrate-resistant acid phosphatase 5 b(TRACP 5 b). Puerarin has a preventive effect in the rat model of disuse osteoporosis and its effect is good, and its mechanism may be related to promoting bone formation and inhibiting bone resorption.

Effect of thrombolytic therapy on intravenous thrombolytic therapy of DNT in acute ischemic stroke and its effect analysis / 中华急诊医学杂志

Objective Analyze the effect of emergency thrombolytic therapy on door to needle time (DNT) in patients with acute ischemic stroke (AIS) and effect.Method Selected 182 cases of AIS patients underwent intravenous thrombolysis at the First Hospital of Qinhuangdao from May 2015 to June 2017.Thrombolytic therapy group (83 cases),for the May 2015-May 2016 after neurological consultation intravenous thrombolysis patients;Emergency thrombolytic group(99 cases),for the June 2016-June 2017 emergency thrombolysis group Emergency Department of intravenous thrombolysis patients.Compare the two groups of DNT,thrombolytic therapy 24 h symptomatic hemorrhage conversion rate,Thrombolysis 24 h,7 dNIHSS score,7 dthrombolysis and 3 months thrombolysis and thrombolysis 3 months improved Rankin score (mRs).Results There was no significant difference in baseline characteristics between the two groups (P＞0.05).Compared with the consultation group,the DNT[(69.77±11.66)min vs (80.12±15.49) min,t=5.745,P ＜ 0.01] of emergency thrombolytic group was significantly shortened,and the good score[39(39.4％) vs 21(25.3％),x2=4.272,P=0.039] at 3 months after treatment was significantly higher (P＜0.05);Treatment of 24 h intracranial hemorrhage conversion rate[12(12.12％) vs 5(6.02％),x2=1.982,P=0.159]、Treatment 7d mortality rate [10(10.10％) vs 6(7.22％),x2=0.464,P=0.496],3 months mortality rate [14(14.14％) vs 11 (13.25％),x2=0.030,P=0.862]、There was no significant difference in the 24h effective rate [57(57.6％) vs 53(63.8％),x2=0.745,P=0.388] and 7d effective rate [50(50.5％) vs 46(55.4％),x2=0.438,P0.508] after treatment (P＞0.05).Conclusions The emergency thrombolytic model can shorten the DNT of rt-PA intravenous thrombolysis in patients with AIS.The safety and efficacy of DNT are not different from the neurological consultation mode,and can improve the good prognosis rate.

Study of texture analysis based on CT images to predict radio-chemotherapy sensitivity in patients with esophageal squamous cell carcinoma / 国际肿瘤学杂志

Objective To investigate the relationship between texture features based on CT and radiochemotherapy sensitivity in patients with esophageal squamous cell carcinoma (ESCC).Methods A total of 92 ESCC patients treated at Shandong Cancer Hospital Affiliated to Shandong University between January 2014 and December 2017 were retrospectively collected.All patients were divided into responders (complete response + partial response) and nonresponders (stable disease + progression disease) according to therapeutic sensitivity.The texture features were extracted from CT images for positioning.And the patients were divided into training set (46 patients) and test set (46 patients) using train-test-split,training set for establishing predictive model and test set for model validation.Results There were 31 responders and 15 nonresponders in the training set,and the portion of responders was 67.4％.Univariate analyses showed that the histogram matrix (HISTO)-sknewess was significantly different between the two groups (Z =2.097,P =0.036) and the area under the curve (AUC) was 0.692 with 95％ CI of 0.539-0.820.Sknewess ≤-2.58 intended to be responders.Binary logistic regression of sknewess (Z =2.097,P =0.036) in HISTO,high gray level zone emphasis (HGZE) (Z =1.722,P =0.085) and small zone high gray level emphasis (SZHGE) (Z =1.640,P =0.101) in gray level zone-length matrix (GLZLM) showed that sknewess was the independent influence factor of sensitivity (OR =0.558,95 ％ CI:0.338-0.923,P =0.023),and the AUC of logistic regression model was 0.718 with 95％ CI of 0.550-0.886,which indicted that the model had the ability to predict treatment response of ESCC patients.The model was validated by using test set and the AUC was 0.706,and the sensitivity of the model was 70.6％ while the specificity was 69.0％.It showed that the model had certain ability in predicting treatment response.Conclusion CT texture analysis can predict the radio-chemotherapy sensitivity in patients with ESCC to some extent.

Effect of atomization inhalation of budesonide and terbutaline sulfate on serum levels of chemokine like factor-1 and cyclooxygenase 2 in children with bronchial asthma / 中国基层医药

Objective To investigate the effect of atomization inhalation of budesonide and terbutaline sulfate on serum levels of cyclcoxygenase 2 (COX-2) and chemokine like factor-1 (CKLF-1) in children with bronchial asthma.Methods A total of 78 children with bronchial asthma in Hangzhou Children's Hospital from April 2016 to August 2017 were selected and divided into the control group(n =39) and the study group(n =39).The control group was treated with routine treatment,and the study group was treated with budesonide and terbutaline sulfate on the basis of the control group.The treatment was continued for 7 d.After t treatment,the clinical effects,clinical symptoms improvement,hospitalization time,the serum levels of COX-2 and CKLF-1 before and after treatment,and the incidence of adverse reaction of two groups were observed.Results The total effective rate of the study group(94.87％) was higher than that of the control group (74.36％)(x2 =6.303,P ＜ 0.05).The improvement time of chest tightness,wheezing,cough and hospitalization time of the study group were shorter than those of the control group (t1 =13.054,t2 =7.365,t3 =4.944,t4 =8.342,all P ＜ 0.05).After treatment,the levels of serum COX-2 and CKLF-1 in the two groups were lower than those before treatment,which of the study group were lower than those in the control group(t1 =4.934,t2 =4.660,all P ＜0.05).There was statistically significant difference in the incidence rate of adverse reactions between the study group(12.82％) and the control group(7.69％) (x2 =0.139,P ＞ 0.05).Conclusion Atomization inhalation of budesonide and terbutaline sulfate in the treatment of children with bronchial asthma can effectively alleviate the clinical symptoms,reduce serum levels of COX-2 and CKLF-1,improve therapeutic effect,promote children's recovery and shorten the hospitalization time.Besides,the incidence rate of adverse reactions is low,and it is safe.